The Effect of a Polyester Nanofibrous Membrane with a Fibrin-Platelet Lysate Coating on Keratinocytes and Endothelial Cells in a Co-Culture System

Chronic wounds affect millions of patients worldwide, and it is estimated that this number will increase steadily in the future due to population ageing. The research new therapeutic approaches wound healing includes development nanofibrous meshes use platelet lysate (PL) stimulate skin regeneration. This study considers a combination degradable electrospun blend poly(L-lactide-co-ε-caprolactone) poly(ε-caprolactone) (PLCL/PCL) membranes (NF) fibrin loaded with various concentrations PL aimed at bioactive dressings. cytocompatibility NF membranes, as well effect PL, was evaluated both monocultures co-cultures human keratinocytes endothelial cells. We determined were able adhere on all their increased proliferation differentiation observed contained least 50% (Fbg + PL) after 14 days. With respect co-culture experiments, 20% enhance metabolic activity cells migration, keratinocytes. results suggest newly developed combined described study, provides promising dressing for chronic purposes.